1/T₁ NMRD profiles of solutions of Mn²⁺ and Gd³⁺ protein‐chelate conjugates

Abstract

Bovine immunoglobulins (IgG) and bovine serum albumin (BSA) were multiply labeled with multidentate ligands, either ethylenediaminetetraacetic acid (EDTA) or diethylene lriaminepentaacetic acid (DTPA), and metal ions were inserted to form the ternary protein ligand‐ion conjugates. The NMRD profiles (the magnetic field dependence of 1/T1) of solutions of the ternary conjugates differ greatly from those of the corresponding binary ligand‐metal‐ion complexes, both in magnitude and functional form, exhibiting 5‐to 10 fold greater relaxivities and prominent peaks near 20 MHz. The inference is that the protein‐bound chelates are relatively rigidly attached to the macromolecules. The structure and metal ion affinities of these novel conjugates, as well as the relevance to contrast enhancement in NMR imaging, is discussed. © 1986 Academic Press, Inc. Copyright © 1986 Wiley‐Liss, Inc., A Wiley Company