A technique for generating initial main-chain atom positions in protein crystallography, using a restrained least squares refinement program

Abstract

RESTRAIN is a least squares refinement program for use on polypeptide and protein structures (Moss & Morffew, 1982). This procedure makes use of the. Severe problems were encountered with the carbonyl oxygens which were finally overcome using a novel technique that placed the oxygens before the first. © 1983.