Nested hydrodynamic flow confinement and liquid recirculation: Microscale probing and patterning of biological surfaces

Abstract

To perform biochemistry at the microscale efficiently, we developed strategies to utilize one or more chemicals simultaneously with the microfluidic probe (MFP). The MFP allows for the manipulation and the "shaping" of nL of multiple liquids, to bring them in contact with a surface or to retrieve a biochemical compound from a surface. Here, we showed the possibility of using multiples flows to shape fluids in the vicinity of surfaces to improve biochemical reagent consumption and perform reconcentration of retrieved analytes by recirculating liquid.